Cytokinesis-block micronucleus assay of celecoxib and celecoxib derivatives
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چکیده
منابع مشابه
Laboratory intercomparison of the cytokinesis-block micronucleus assay.
The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1-6.4 Gy) were sent to the participants. The CBM...
متن کاملTesting chemical agents with the cytokinesis-block micronucleus cytome assay.
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimeti...
متن کاملMicronucleus assay of human lymphocytes: a comparison of cytokinesis-block and human capillary blood lymphocytes methods.
Micronucleus (MN) expression in Peripheral blood lymphocytes is well established as a method to monitor chromosome damage in human populations. The use of MN as a measure of chromosome damage in peripheral blood lymphocytes was first proposed by Countryman and Heddle and subsequently improved with the development of the cytokinesis-block micronucleus (CBMN) method, which has been extensively us...
متن کاملApplication of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay.
Among the numerous methods available to assess genotoxicity, the cytokinesis-block micronucleus (CBMN) assay is very popular due its relative simplicity and power to detect both clastogenic and aneugenic compounds. A problem with the CBMN assay is that all DNA damaging agents also inhibit the ability of cells to progress through mitosis, leading to a low number of binucleated cells (BNCs). One ...
متن کاملThe cytokinesis-block micronucleus assay: experimental procedure and application in bioelectromagnetics research
The cytokinesis-block micronucleus assay is a sensitive and simple indicator of chromosome damage, both chromosome loss and chromosome breakage, which also provides information on cell cycle progression and cytotoxicity. It was introduced in 1985 and has been successfully employed in many laboratories on different cell types to monitor exposed population, to identify mutagen-sensitive individua...
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ژورنال
عنوان ژورنال: Toxicology Reports
سال: 2020
ISSN: 2214-7500
DOI: 10.1016/j.toxrep.2020.11.004